Job Description
Job Description
Responsibilities:
- Perform basic cell line development (CLD) techniques and generate stable cell lines using transfection, transduction, selection, single cell subcloning, growth and expression screening, cell banking and stability assessment.
- Support molecular and genetic characterization of producer cell lines and leverage these findings for the development of novel cell line substrates.
- Contribute towards the design and implementation of new technologies and genome engineering strategies to support CLD platform innovation.
- Maintain accurate and detailed laboratory notebooks and electronic records.
- Interpret project results and contribute to technical discussions by effectively communicating key updates to collaborators and team members.
- Capability to work independently and manage multiple projects.
- Highly organized with strong attention to detail, accuracy, and conciseness.
- Excellent analytical and problem-solving skills.
- Strong planning skills and a history of meeting accelerated timelines.
- Demonstrate a strong sense of work ethic and agility in a dynamic, cross-functional, collaborative environment.
Basic Qualification:
- Ph.D., MS, or BS degree in Biochemistry, Cell Biology, Molecular Biology, Bioengineering or relevant fields with 0-2yr (PhD), 2-4yr (MS), 3-5yr (BS) years of relevant experience within a pharmaceutical or biotech industry.
- Extensive experience with mammalian cell culture (adherent and suspension) with strong aseptic techniques required.
- Hands-on experience in Cell Line development is preferred
- Proven expertise in molecular biology assays such as DNA/RNA extraction, PCR, Sanger Sequencing, qPCR/ddPCR/dPCR for viral titer, copy number and transcript analysis, Western blot, Flow cytometry required. Hands-on experience with laboratory instrumentation such as JESS, WES, Attune NxT, Chemometec NC3000, Refeyn, Incucyte, VIPS single-cell seeder is preferred.
- Experience in upstream processes like virus production, purification, and characterization protocols preferred.
- Experience using genome editing tools (CRISPR and transposases) to modify mammalian cell lines preferred.
- Experience analyzing some/all the following platforms: whole-genome/exome sequencing, RNA-sequencing (e.g., Illumina), single-cell/nucleus RNA-seq (10x Genomics), High-throughput library-based discovery, and long-read sequencing (e.g., PacBio and Oxford Nanopore)
- Proficiency with Microsoft Office Suite and GraphPad Prism, and familiarity with additional software such as SW compass, QuantStudio, Snapgene, and GeneiousPrime.
“Nice-to-have” Qualifications:
- Familiarity with biological annotation and ontology resources, such as Ensembl, GO, and UniProt
- Experience using Java, JavaScript, Python, R, Scala, or SQL to organize and analyze complex data sets
- A background in neuroscience and/or virology
Company DescriptionThe Steely Group is a national preclinical, clinical, and commercial staffing firm and certified women’s business enterprise. We partner with small, growing biotech, pharma, and gene therapy companies to help bring treatments to patients by providing flexible talent acquisition solutions including Contingent Search (Direct Hire & Contract Consultants) and Retained Executive Search.
Company Description
The Steely Group is a national preclinical, clinical, and commercial staffing firm and certified women’s business enterprise. We partner with small, growing biotech, pharma, and gene therapy companies to help bring treatments to patients by providing flexible talent acquisition solutions including Contingent Search (Direct Hire & Contract Consultants) and Retained Executive Search.